TB-500: Research, Dosing, and Where to Buy in 2026
A research-first profile for TB-500 (Thymosin Beta-4 fragment, TB500), aggregating mechanism notes, transcript dosing mentions, vendor recommendations, and PubMed-indexed literature without presenting medical guidance.
Synthetic fragment of the natural protein Thymosin Beta-4. As of July 2026, The Peptide Wiki aggregates 8 PubMed-cited papers, 22 attributed expert mentions, 22 transcript dosing protocols, and 5 referenced vendors for TB-500, each linked to its source.
- Also indexed as Thymosin Beta-4 fragment, TB500.
- Synthetic fragment of the natural protein Thymosin Beta-4. Most-known peptide for soft tissue + cardiac repair research.
- Mechanism notes include Binds G-actin, promotes cell migration during tissue repair. Upregulates VEGF and stem cell mobilization.
- 22 aggregated dosing-protocol mentions from source transcripts.
- 8 PubMed-indexed citations listed in the research table below.
- 5 vendor recommendations captured from named experts.
- Evidence level in this entry is marked as research.
What is TB-500?
Synthetic fragment of the natural protein Thymosin Beta-4. Most-known peptide for soft tissue + cardiac repair research.
Experts on background and overview · 11 cited mentions
"TB-500 BPC-157 KPV 2 milligrams of each every single day. And GK copper 10 milligrams per day. That's um separately now, but I'll get some close stacks and then just do one single shot."
"TB-500 twice per day, BPC-157 twice per day. I don't know the growth stack that's on the way. I do like 12 injections twice per day."
"Combine that with 50 mg to 100 mg of a dipeptidyl peptidase 4 inhibitor, so you prevent the breakdown of BPC and TB-500."
Show 8 more cited mentions on background and overview →
"1 mg TB-500 and BPC-157 morning and evening. Do you use growth hormone a couple times per day or MK-677"
"I would wait with BPC and TB-500 until you've been cleared because for tumors and cancer, you probably don't want angiogenesis... these kinds of growth factors like TB-500, BPC-157, growth hormone, maybe... TB-500, and certainly growth hormone and its downstream hormone IGF-1 can be um quite prolific."
"Steve reported that GHK, BPC-157, and TB-500 applied topically may support skin appearance more than typical over-the-counter products, describing them as working 'from the inside.' (Speaker observation, not medical advice.)"
"TB-500 there's five studies showing that it has the same effects as thymosin beta 4 and then you you read the studies on thymosin beta 4 and there's 25,000 studies."
"raise your ariththropetin levels and your insulin levels and your IGF-1 levels with thymosin alpha 4 thymosin alpha 1, right?"
"TB-500 BPC-157 growth hormone Increlex IGF-1 LR3 [cancer-promoting growth peptides to avoid in cancer-risk clients]."
"GI aids such as oral BPC, oral TB, uh KPV."
"BPC-157, TB-500 for soft tissue healing."
How does TB-500 work?
Binds G-actin, promotes cell migration during tissue repair. Upregulates VEGF and stem cell mobilization.
Mechanism note
These mechanisms are presented as research observations and hypotheses from the indexed data, not as confirmation of clinical effect in humans.
What is TB-500 researched for?
Accelerates wound and tissue healing, especially in muscle, tendon, and skin. Often co-administered with BPC-157.
Experts on uses and effects · 1 cited mention
"I did nine weeks, eight weeks of TB-500 as well as I mean I went to Beverly Hills surgeons... I had what is called a sports hernia... a tendon from my groin. It's like torn off of my pelvis."
TB-500 Dosing Protocols
The entries below are transcript-derived dosing mentions. They are preserved for research context and are not medical advice.
| Speaker | Source | Timestamp | Protocol | Evidence |
|---|---|---|---|---|
| Vigorous Steve | Staying Dry On A Cut, Injectable Oral AAS, Sleep: GH Secretagogues... | 01:07:55 | Steve reported (anecdotal) using TB-500 twice per day as part of a quad-tear recovery stack alongside BPC-157, GHK-Cu, and KPV, continuing until strength returned. Educational reporting only - not medical advice; research compound, not for human consumption. | anecdotal |
| Hunter Williams | The TB-500 Masterclass | Complete User's Guide (2026) | 00:15:13 | Hunter Williams reported the following protocol for severe or complex injury: 5 mg twice per week for first 2 weeks, then 2 to 2.5 mg twice per week for 4 weeks, then 2 mg once per week for 4 to 6 weeks maintenance; total cycle 10 to 12 weeks. | anecdotal |
| Vigorous Steve | FULL Quad Tear Recovery Protocol, HGH Serum Tests, Maximize Cardio... | 00:05:48 | Steve reported using 2 mg TB-500 daily as a localized injection at the site of injury (anecdotal, source video IwJ7q0Fae9I). Research compound - not for human consumption; educational only. | anecdotal |
| Andrew Williams | The KPV Masterclass | Complete User's Guide (2026) | 00:21:02 | Injury stack reported by Andrew Williams: subcutaneous KPV at 500 mcg daily, layered with standard BPC plus TB-500, for a reported duration of 6 to 12 weeks or until healing progressed. | anecdotal |
| Vigorous Steve | Nebido For Traveling, Skin-Friendly PEDs, GH Carpal Tunnel, Freeze... | 00:32:00 | 1 mg TB-500 morning and 1 mg evening combined with BPC-157, GH, MK-677, collagen peptides, vitamin C, hyaluronic acid, zinc and Anavar+TRT for injury healing | anecdotal |
| Hunter Williams | The BPC-157 Masterclass | Complete Users Guide (2026) | 00:17:02 | Speaker reports benefit from higher TB500 doses up to about 5 mg/day, while contrasting that with BPC's apparent ceiling around 1 mg/day for him. | anecdotal |
| Vigorous Steve | Underdosed Test Epidemic, Tren Sleep Aids, High-Dose GH & Low-Dose... | 00:56:11 | Vigorous Steve reported using 1 mg TB-500 twice per day (morning and evening) for post-surgical healing (anecdotal, not medical advice). | anecdotal |
| Hunter | Saturday Morning Coffee Talk 6/6/26 | 00:46:25 | For fingertip injury, Taylor suggests 2 mg TB-500; Hunter frames it as high-dose systemic subcutaneous TB-500 for stem-cell migration. | anecdotal |
| Vigorous Steve | (Not Really) Enhanced Games REVIEW! Point Proven, Or Major Flop?... | 01:07:53 | Post-surgical quad tendon repair: TB-500 + BPC-157 stack alongside growth hormone 2.5 IU AM / 2.5 IU PM | anecdotal |
| Vigorous Steve | Injectable SLU, AI Dosing Protocol, Glutathione & NAD+, GH & Insulin... | 00:20:32 | Steve reported using: 1 mg TB-500 bilaterally combined with 1 mg BPC-157 and 2 mg Cartilax | anecdotal |
| Vigorous Steve | Debunking Growth Hormone Myths, Insulin Resistance, Fat Loss,... | 01:56:23 | Year-round usage with BPC-157 for inflammation; take before bed with GH rather than pre-workout | anecdotal |
| Will (JD Denham co-host) | Peptide of the Week: PE-22-28, pinealon & Cerebrolysin - Mood,... | 00:04:16 | 8-9 weeks of TB-500 (paired with hip mobility work) resolved a 10-year sports hernia. | anecdotal |
| Nyle Nayga | Milos Debates PED's, IGF-1, Insulin, Training, & GH | 00:35:55 | Both injectable (soft tissue healing) and oral (GI aid) forms used during prep | anecdotal |
| The Biohacking Specialist | On TRT? Copy this Peptide Stack to Heal Your Gut 10x Faster in 2026... | 00:05:28 | Users have reported a protocol of twice-weekly subcutaneous injections on Monday and Thursday, run for 12 weeks. Some reported cycling off after week 12; others continued with 250 mg once weekly for maintenance. | anecdotal |
| Dr. Jones DC | The Glow Stack Protocol #longevity #healthoptimization | 00:00:47 | Included as part of a clinic 'glow stack' with GHK copper, BPC-157, and TB-500 for anti-aging, recovery, and inflammation reduction; no dose, route, frequency, or cycle length stated. | anecdotal |
| Dr. Jones DC | Wolverine Protocol Didn't Work. Here's Why #peptidetherapy #injuryrecovery | 00:00:29 | Speaker reported discussing TB-500 as part of a properly dosed Wolverine injury cycle, noting that recovery from serious tissue damage requires a 3-month cycle rather than 3 weeks. | anecdotal |
| Dr. Jones DC | The Most Talked-About Peptide Combo #peptides #regenerativemedicine | 00:00:56 | Pharmaceutical grade at therapeutic doses; pharmacy grade from a 503A compounding facility specified as the standard. No specific mg dose or injection frequency given in this clip. | anecdotal |
| VigorousClips | Always Combine Your BPC-157 With This For The Best Results... (It's... | 00:04:06 | Common injury protocol described by speaker_1 (source: 3MSSSxeqE9k): 500 mcg to 1 mg TB-500 with 500 mcg to 1 mg BPC-157, injected as close to the injury site as possible. | anecdotal |
| Boost Your Biology (Lucas Aoun) | Stop Taking Peptides Until You Hear This! | 00:07:16 | Speaker 1 reported using approximately 2 to 2.5 mg twice per week as loading, then dropping to about 1 to 2 mg per week for maintenance. | anecdotal |
| The Biohacking Specialist | Stop using "GLOW" Blends in 2026 (Do this before it's too late) | 00:08:42 | Reported protocol from host: loading phase weeks 1-2 at 5 mg twice per week; maintenance phase weeks 3-12 at 5 mg once per week. | anecdotal |
| The Hunter Williams Podcast | The KPV Masterclass | Complete User's Guide (2026) | 00:27:51 | Sequencing for injury: start KPV 500 mcg/day subcutaneous for the first week, then add BPC and TB-500; reassess by 12 weeks. | anecdotal |
| The Hunter Williams Podcast | Saturday Morning Coffee Talk 6/20/26 | 00:40:34 | Taylor reports most relief for plantar fasciitis/inflamed foot using 2 mg per day TB500/TD500. | anecdotal |
Where to Buy TB-500 in 2026
APR Health Solutions
Code: NYLE (per Nyle's podcast affiliate)Visit vendor → View profile →Recommended by Tanner Tattered at 00:44:06.
APR Health Solutions
Code: NYLE (per Nyle's podcast affiliate)Visit vendor → View profile →Recommended by Nyle at 00:34:26.
APR Health Solutions
Code: NYLE (per Nyle's podcast affiliate)Visit vendor → View profile →Recommended by Niles at 00:35:12.
APR Health Solutions
Code: NYLE (per Nyle's podcast affiliate)Visit vendor → View profile →Recommended by Promo voice at 00:24:58.
Optimize HRT
Code: NYLEVisit vendor → View profile →Recommended by Host at 01:53:51.
Side Effects and Safety
Anecdotal reports and study-level observations vary by route, dose, and individual. This page does not provide medical advice. Consult a qualified clinician before any research use.
Experts on side effects and safety · 1 cited mention
"I do want to add some growth hormone and I do want to add some TB-500 or BPC-157... if you have a little bit of underlying cancer could be a problem, you might accelerate the growth thereof faster with growth hormone and angioenesis producing compounds."
What Experts Say
3 experts across our source library discuss TB-500. Each entry is that expert’s most context-rich mention: click to watch it at the exact timestamp in the original video. Quotes are also emitted as Schema.org Quotation JSON-LD for AI assistants.
-
“TB-500 BPC-157 KPV 2 milligrams of each every single day. And GK copper 10 milligrams per day. That's um separately now, but I'll get some close stacks and then just do one single shot.”
-
“GI aids such as oral BPC, oral TB, uh KPV.”
-
“I did nine weeks, eight weeks of TB-500 as well as I mean I went to Beverly Hills surgeons... I had what is called a sports hernia... a tendon from my groin. It's like torn off of my pelvis.”
See 10 more expert mentions →
-
“TB-500 twice per day, BPC-157 twice per day. I don't know the growth stack that's on the way. I do like 12 injections twice per day.”
-
“Combine that with 50 mg to 100 mg of a dipeptidyl peptidase 4 inhibitor, so you prevent the breakdown of BPC and TB-500.”
-
“1 mg TB-500 and BPC-157 morning and evening. Do you use growth hormone a couple times per day or MK-677”
-
“I would wait with BPC and TB-500 until you've been cleared because for tumors and cancer, you probably don't want angiogenesis... these kinds of growth factors like TB-500, BPC-157, growth...”
-
“Steve reported that GHK, BPC-157, and TB-500 applied topically may support skin appearance more than typical over-the-counter products, describing them as working 'from the inside.' (Speaker...”
-
“TB-500 there's five studies showing that it has the same effects as thymosin beta 4 and then you you read the studies on thymosin beta 4 and there's 25,000 studies.”
-
“raise your ariththropetin levels and your insulin levels and your IGF-1 levels with thymosin alpha 4 thymosin alpha 1, right?”
-
“TB-500 BPC-157 growth hormone Increlex IGF-1 LR3 [cancer-promoting growth peptides to avoid in cancer-risk clients].”
-
“BPC-157, TB-500 for soft tissue healing.”
-
“I do want to add some growth hormone and I do want to add some TB-500 or BPC-157... if you have a little bit of underlying cancer could be a problem, you might accelerate the growth thereof faster...”
User Reviews
User reviews aggregator coming Q3 2026 (Reddit + YouTube comments + Discord research notes). Until then, see the expert quote section above and PubMed citations below.
Research Efficacy Snapshot
Published efficacy percentages cited in PubMed trials and named-expert reports about TB-500. Each line links to the original source.
- "and the last one is 50% off and that is for BPC57, TB500, Milanotan 2, Mati, SS31 and I think it's also valid for the CLO and the Glow stacks" · Vigorous Steve / Steve on "Prevent Insulin Resistance On High-Dose GH, Mirabegron Vs. C" @ 1:26
- "So, I would wait until you're 100% tumor free." · Steve on "Oral Vs Injectable SLU-PP-332, Ideal GH To Test Ratio, High " @ 1:32:16
Research and Studies
8 PubMed-indexed papers reference TB-500. Top 8 shown.
Legal Status
TB-500 is presented here as a research compound. FDA approval status, scheduling, WADA status, and state-specific telehealth rules may apply. Always verify current regulatory status. Last verified: 2026-07-09.
Frequently Asked Questions
What is TB-500?
Synthetic fragment of the natural protein Thymosin Beta-4. Most-known peptide for soft tissue + cardiac repair research.
Is TB-500 FDA-approved?
TB-500 is presented on this page as a research compound. FDA approval status, scheduling, and state-specific rules may apply and change. Verify current regulatory status before any decision.
What dosing is reported for TB-500?
This page aggregates 22 dosing-protocol mention(s) from named-expert transcripts, each linked to a verbatim source timestamp. See the Dosing Protocols table above. Mentions are anecdotal, not medical advice.
Where can I buy TB-500?
5 vendor recommendation(s) are tracked in our directory, sourced from named-expert mentions. See the Where to Buy section above. Always verify COA and current regulatory status.
How much research has been published on TB-500?
8 PubMed-indexed paper(s) reference TB-500 in our current research feed. See the Research and Studies section above for citations.